Differentiating vector-derived mRNA from contaminating DNA templates by inverse RT-PCR.
نویسندگان
چکیده
Inverse RT-PCR was used for detecting doxycycline-induced mRNA expression after viral transduction with a retroviral vector harboring human TDAG51. After the circularization of double-stranded cDNA, induced transcripts originating from integrated vector genomes were selectively amplified, even in the presence of DNA templates. Thus, DNase treatment before amplification was unnecessary.
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عنوان ژورنال:
- BioTechniques
دوره 31 6 شماره
صفحات -
تاریخ انتشار 2001